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primary antibodies against βactin  (Millipore)


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    Millipore primary antibodies against βactin
    Primary Antibodies Against βactin, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+antibodies+against+%CE%B2actin/%CE%B2+actin+antibody/pmc11447051__12276_2024_1300_MOESM1_ESM-74-24-29
    Average 90 stars, based on 1 article reviews
    primary antibodies against βactin - by Bioz Stars, 2026-09
    90/100 stars

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    Blocking Assay:

    Article Title: Crosstalk between FTH1 and PYCR1 dysregulates proline metabolism and mediates cell growth in KRAS -mutant pancreatic cancer cells
    Article Snippet: .. Page 9 blocking them with 5% skim milk in Tris-buffered saline containing 0.1% Tween-20 (TBST) for 30 min, we incubated the membranes with primary antibodies against βactin (1:7500; #A1978, Millipore), α-tubulin (1:7500; #A6830, ABclonal), GAPDH (1:7500; #A10868, ABclonal), pERK1/2 (1:1000; #9101, Cell Signaling Technology), total Erk1/2 (1:2000; #E-AB-12397, Elabscience), FTH1 (1:2500; #CPA4412, Cohesion), FTL (1:500; #LS‐C212054, LSBio), PYCR1 (1:7500; #20962-1-AP, Proteintech), PYCR2 (1:7500; #CQA5644, Cohesion), and PRODH (1:2000; #CQA1282, Cohesion) overnight. .. The membranes were then washed with TBST for 5 min three times, followed by incubation with appropriate horseradish peroxidase– conjugated secondary antibodies at room temperature for 1 h. Protein bands were detected using the electrochemiluminescence reagent (WBLUF0500; Millipore) and imaged and assessed using the UVP biochemical system and VisionWorks LS (VisionWorks).

    Saline:

    Article Title: Crosstalk between FTH1 and PYCR1 dysregulates proline metabolism and mediates cell growth in KRAS -mutant pancreatic cancer cells
    Article Snippet: .. Page 9 blocking them with 5% skim milk in Tris-buffered saline containing 0.1% Tween-20 (TBST) for 30 min, we incubated the membranes with primary antibodies against βactin (1:7500; #A1978, Millipore), α-tubulin (1:7500; #A6830, ABclonal), GAPDH (1:7500; #A10868, ABclonal), pERK1/2 (1:1000; #9101, Cell Signaling Technology), total Erk1/2 (1:2000; #E-AB-12397, Elabscience), FTH1 (1:2500; #CPA4412, Cohesion), FTL (1:500; #LS‐C212054, LSBio), PYCR1 (1:7500; #20962-1-AP, Proteintech), PYCR2 (1:7500; #CQA5644, Cohesion), and PRODH (1:2000; #CQA1282, Cohesion) overnight. .. The membranes were then washed with TBST for 5 min three times, followed by incubation with appropriate horseradish peroxidase– conjugated secondary antibodies at room temperature for 1 h. Protein bands were detected using the electrochemiluminescence reagent (WBLUF0500; Millipore) and imaged and assessed using the UVP biochemical system and VisionWorks LS (VisionWorks).

    Incubation:

    Article Title: Crosstalk between FTH1 and PYCR1 dysregulates proline metabolism and mediates cell growth in KRAS -mutant pancreatic cancer cells
    Article Snippet: .. Page 9 blocking them with 5% skim milk in Tris-buffered saline containing 0.1% Tween-20 (TBST) for 30 min, we incubated the membranes with primary antibodies against βactin (1:7500; #A1978, Millipore), α-tubulin (1:7500; #A6830, ABclonal), GAPDH (1:7500; #A10868, ABclonal), pERK1/2 (1:1000; #9101, Cell Signaling Technology), total Erk1/2 (1:2000; #E-AB-12397, Elabscience), FTH1 (1:2500; #CPA4412, Cohesion), FTL (1:500; #LS‐C212054, LSBio), PYCR1 (1:7500; #20962-1-AP, Proteintech), PYCR2 (1:7500; #CQA5644, Cohesion), and PRODH (1:2000; #CQA1282, Cohesion) overnight. .. The membranes were then washed with TBST for 5 min three times, followed by incubation with appropriate horseradish peroxidase– conjugated secondary antibodies at room temperature for 1 h. Protein bands were detected using the electrochemiluminescence reagent (WBLUF0500; Millipore) and imaged and assessed using the UVP biochemical system and VisionWorks LS (VisionWorks).



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